134IN-VITRO DETERMINATION OF SUN PROTECTION FACTOR AND EVALUATION OF HERBAL OILSThe aim of this study was to determine the Sun Protection Factor of Herbal oil that can be used in sunscreen formulation. Herbal phytoconstituents act as antioxidant which reflect or absorbed UV- radiation before reaching to the skin. Sun protection factor is determined by spectrophotometric method according to Mansur et al in the UV region 290-320 nm. From the result it was found that SPF of carrot seed oil was found to be 19, SPF of wheat germ oil was found to be 22, SPF of jojoba oil was found to be 06 and SPF of olive oil was found to be 09.Higher the SPF value higher will be the UV absorption activity of sunscreen. All this oils have been used in cosmetic for various purposes like emollient, antioxidants, anti-ageing and anti-wrinkle. In future this study will be helpful for selection of herbal oil for the development of sunscreen formulation that gives better potential sunscreen effect if they would be used in combination then might it will be given synergistic sunscreen effect.saonereja@gmail.comResearch612016Sun Protection Factor,Herbal oilJyotsna A Saonere SuryawanshiDepartment of Pharmacy, Govt. Polytechnic, Amravati, Maharashtra, India